ANALYSIS
Analysing Your Own Mutation Data for Clusters
| Coloum Number | Name | State | Description |
|---|---|---|---|
| 1 | Hugo_Symbol | Standard | Hugo_symbols refer to the naming of human genes from the HUGO Gene Nomenclature Committee. |
| 2 | Chromosome | Standard | Chromosome field is a numeric value, for example, 18 indicates that the mutation appears on chromosome 18. |
| 3 | Start_Position | Standard | The starting position. |
| 4 | End_Position | Standard | The end position. |
| 5 | Varient_classfication | Standard | No need to screen for mutation types provided. Any varriant classfications can for HOTSPOT3D, not just missense mutation. |
| 6 | Reference_Allele | Standard | Reference allele refers to the base that is found in the reference genome. |
| 7 | Reference_Allele1 | Standard | |
| 8 | Reference_Allele2 | Standard | |
| 9 | Tumor_Sample_Barcode | Standard | |
| 10 | Transcript_name | Non-standard | A transcript ID column, referring to Ensembl coding transcript ID's (ENST). |
| 11 | amino_acid_change | Non-standard | A protein peptide change column (HGVS p. single letter abbreviations, ie p.T790M) |
DATABASE
HotSpot3D, to identify spatial hotspots (clusters) and to interpret the potential function of variants within them. We applied HotSpot3D to >4,400 TCGA tumors across 33 cancer types, and obtained the following results:
Clusters
Druggable Mutations
Rare Mutations
Medium Reccurence Mutations
HotSpot3D
Hotspot3D is a high-precision software for identifying high-frequency mutation hotspots in pan-cancer somatic cells.
Accessibility
Hotspot3D is a high-precision software for identifying high-frequency mutation hotspots in pan-cancer cells. HotSpot3D Web is dedicated to providing researchers with an easier way: you can quickly get software results using the HotSpot3D kernel with a single click of the prepared mutant data file.
Visualization and Re-visualization
Hotspot3D Web provides you with result data files and 3D visualization of the mutation hotspots. In addition, re-visualization is supported. Anytime, you can view mutation hotspots on protein 3D structure by uploading the results file.
Sharing
We applied Hotspot3D to TCGA to >4,400 TCGA tumors across 19 cancer types including BLCA, BRCA, CESC and so on. We directly provide results and visualization of clustering data on mutations here .